Journal: PloS one

Article Title: Embedding synthetic microvascular networks in poly(lactic acid) substrates with rounded cross-sections for cell culture applications.

doi: 10.1371/journal.pone.0073188

Figure Lengend Snippet: Figure 3. Seeding and culture of bovine aortic endothelial cells (BAECs) throughout PLA microchannel networks. (a) Confocal microscopy shows that the interior walls of a 200 mm diameter straight circularized microchannel can be uniformly seeded with endothelial cells that subsequently are confluently cultured in a monolayer lining the channel wall. (b) Fluorescent images show BAECs survive and maintain their morphology after 5 days in the straight circularized microchannel (bar, 50 mm). (c) BAECs seeded in four generations of branched microchannel network with diameters extending below 50 mm uniformly cover all channel walls and maintain viability after 3 days of culture (bar, 50 mm). doi:10.1371/journal.pone.0073188.g003

Article Snippet: Bovine Aortic Endothelial Cells (BAECs; Cell Applications, Inc., CA) obtained as a kind gift from Dr. Mariah Hahn at Texas A&M University were resuspended at 56106 cells mL–1 medium for 500 mm diameter channels or at 26107 cells mL–1 medium for 100 mm diameter and branched channels.

Techniques: Confocal Microscopy, Cell Culture

Journal: Glycobiology

Article Title: The Lec5 glycosylation mutant links homeobox genes with cholesterol and lipid-linked oligosaccharides

doi: 10.1093/glycob/cwy103

Figure Lengend Snippet: Properties of Lec5 and Lec5Rev cells. LLOs were analyzed by fluorophore-assisted carbohydrate electrophoresis (Gao and Lehrman 2006; Gao et al., 2013). (A) G3M9-LLO in Lec5 cells increases with cell density (L, low; M, medium; H, high). A representative gel, and a graph of G3M9-LLO quantity is shown. (B) G3M9-LLO is elevated in Lec5Rev cells (Rev). For comparison, LLOs in Lec5 (M5-LLO) and Lec15 (M5-LLO, G3M5-LLO) mutants are shown. All LLOs were sensitive to 2 h treatments with 5 μg/mL tunicamycin (TN). (C) LLOs of Lec5 subclones and revertants used for RNA-Seq.

Article Snippet: We reassessed LLO synthesis in validated Lec5 (B211) cells ( Stanley 1983 ) in comparison with the CHO wild-type line designated CHO-K1 (ATTC CCL-61; which is distinct from the CHO wild-type line designated Pro-5, ATCC-1781 ( Stanley 1983 )), using the non-radioactive method “Fluorophore-Assisted Carbohydrate Electrophoresis” ( Gao and Lehrman 2006 ; Gao et al., 2013 ) (Figure ).

Techniques: Electrophoresis, Comparison, RNA Sequencing

Journal: Glycobiology

Article Title: The Lec5 glycosylation mutant links homeobox genes with cholesterol and lipid-linked oligosaccharides

doi: 10.1093/glycob/cwy103

Figure Lengend Snippet: Top canonical pathways by IPA ® (Lec5 VS Lec5 Rev ) a

Article Snippet: We reassessed LLO synthesis in validated Lec5 (B211) cells ( Stanley 1983 ) in comparison with the CHO wild-type line designated CHO-K1 (ATTC CCL-61; which is distinct from the CHO wild-type line designated Pro-5, ATCC-1781 ( Stanley 1983 )), using the non-radioactive method “Fluorophore-Assisted Carbohydrate Electrophoresis” ( Gao and Lehrman 2006 ; Gao et al., 2013 ) (Figure ).

Techniques: Indirect Immunoperoxidase Assay, Significance Assay, Activation Assay

Journal: Glycobiology

Article Title: The Lec5 glycosylation mutant links homeobox genes with cholesterol and lipid-linked oligosaccharides

doi: 10.1093/glycob/cwy103

Figure Lengend Snippet: Top transcript hits and fold-increases in Lec5

Article Snippet: We reassessed LLO synthesis in validated Lec5 (B211) cells ( Stanley 1983 ) in comparison with the CHO wild-type line designated CHO-K1 (ATTC CCL-61; which is distinct from the CHO wild-type line designated Pro-5, ATCC-1781 ( Stanley 1983 )), using the non-radioactive method “Fluorophore-Assisted Carbohydrate Electrophoresis” ( Gao and Lehrman 2006 ; Gao et al., 2013 ) (Figure ).

Techniques: